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Digital books on light and electron microscopy

Biomedical Technology (Lecture Notes in Applied and Computational Mechanics Book 74) 2015 Edition, Kindle Edition

During the last years computational methods lead to new approaches that can be applied within medical practice. Based on the tremendous advances in medical imaging and high-performance computing, virtual testing is able to help in medical decision processes or implant designs. Current challenges in medicine and engineering are related to the application of computational methods to clinical medicine and the study of biological systems at different scales.
Additionally manufacturers will be able to use computational tools and methods to predict the performance of their medical devices in virtual patients. The physical and animal testing procedures could be reduced by virtual prototyping of medical devices. Here simulations can enhance the performance of alternate device designs for a range of virtual patients. This will lead to a refinement of designs and to safer products.
This book summarizes different aspects of approaches to enhance function, production, initialization and complications of different types of implants and related topics.
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Cell Biological Applications of Confocal Microscopy (Methods in Cell Biology Book 70) 2nd Edition, Kindle Edition

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.

An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM).

  • Includes detailed protocols accessible to new users
  • Details how to set up and run a "Confocal Microscope Core Facility"
  • Contains over 170 figures
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Digital Microscopy: 113 (Methods in Cell Biology) 3rd Edition, Kindle Edition

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
  • Expands coverage to include discussion of confocal microscopy not found in the previous edition
  • Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
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Digital Microscopy: A second edition of "Video Microscopy" (Methods in Cell Biology Book 72) 2nd Edition, Kindle Edition

This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half.
  • Shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy
  • Contains 10 new chapters addressing developments over the last several years
  • Covers basic principles, applications, and equipment
  • Spcialized video microscopy techniques are covered
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Digital Microscopy (Methods in Cell Biology Book 114) 4th Edition, Kindle Edition

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
  • Expands coverage to include discussion of confocal microscopy not found in the previous edition
  • Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
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Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique (RMS - Royal Microscopical Society) 1st Edition, Kindle Edition

Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty.

The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques.

The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.
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Environment Exposure to Pollutants (Advances in Experimental Medicine and Biology Book 834) 2015 Edition, Kindle Edition

The impairment of lung function caused by environmental exposure to pollutants and toxicants is a rising health problem, particularly in highly industrialized parts of the world. The problem is urgently calling for the development of new methodologies to assess both the level of elemental exposure and the effects for quality of health and longevity. This volume provides state-of-the-art information about the recent advances in occupational and non-occupational pollutant-related disorders of the respiratory tract, and the assessment of a threat they pose for the health-span. Heavy traffic-related air pollution, unnoticeable but salient health detriment, is dealt with at length.
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Essential Environment: The Science behind the Stories 5th Edition, Kindle Edition


Essential Environment: The Science behind the Stories, Fifth Edition engages students using current, integrated case studies that provide a context for understanding science and environmental concerns in a brief, 18-chapter text. Jay Withgott and Matt Laposata present the latest understanding of environmental science along with expanded FAQ discussions that address common student misconceptions and with new Data Analysis questions that build quantitative literacy.
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Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis (Biological Techniques Series) 2nd Edition, Kindle Edition

The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.
The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.

TOPICS COVERED:
  • Single and multiphoton confocal microscopy
  • Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies
  • Applications of new optical probes for imaging proteins in gels
  • Probes and detection technologies for imaging membrane potential in live cells
  • Use of optical probes to detect microorganisms
  • Raman and confocal raman microspectroscopy
  • Fluorescence lifetime imaging microscopy
  • Digital CCD cameras and their application in biological microscopy
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Fluorescence Microscopy In Life Sciences Kindle Edition

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
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Fluorescence Microscopy: Super-Resolution and other Novel Techniques 1st Edition, Kindle Edition

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers.

With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research.

  • Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research
  • Offers insight into common challenges in implementing techniques, as well as effective solutions
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Fundamentals of Light Microscopy and Electronic Imaging 2nd Edition, Kindle Edition

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.

The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.

PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website:
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Force Microscopy: Applications in Biology and Medicine 1st Edition, Kindle Edition

This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function.

The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers:
  • Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland
  • Probing the structural and physical properties of microbial cell surfaces
  • Scanning probe microscopic characterization of the higher plant cell wall and its components
  • Case studies of nano drug delivery systems using engineered dendrimers
  • AFM techniques for studying living cells
  • Investigating the intermolecular forces of leukocyte adhesion molecules
  • Protein-protein interactions
  • Micromechanical properties of lipid bilayers and vesicles

The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine.

This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.
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Light and Video Microscopy 1st Edition, Kindle Edition

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

  • Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope 
  • Clearly develops all ideas from historical and logical foundations 
  • Laboratory exercises included to assist the reader with practical applications 
  • Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope
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Low Voltage Electron Microscopy: Principles and Applications (RMS - Royal Microscopical Society) 1st Edition, Kindle Edition

Part of the Wiley-Royal Microscopical Society Series, this book discusses the rapidly developing cutting-edge field of low-voltage microscopy, a field that has only recently emerged due to the rapid developments in the electron optics design and image processing.

It serves as a guide for current and new microscopists and materials scientists who are active in the field of nanotechnology, and presents applications in nanotechnology and research of surface-related phenomena, allowing researches to observe materials as never before.
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Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy 2002 Edition, Kindle Edition

Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs.

This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
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Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension 2011 Edition, Kindle Edition

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation
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Optical Imaging Techniques in Cell Biology 1st Edition, Kindle Edition

Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope’s technical progression happened in a series of fits and starts until the late 19th century. After Ernst Abbe perfected the “how” of lens design, achieving the theoretical limit imposed by wavelength, there came a revolution in subject matter or “what” could be studied by microscope.

Covering the entire field of microscopy, Optical Imaging Techniques in Cell Biology provides an overview of the technical evolution of the microscope and explains how the basics of optical microscopy led to the most advanced techniques employed today. The author addresses a vast array of topics including optical contrasting techniques, fluorescence, confocal versus widefield microscopes, lasers as a light source, and digital imaging, as well as the correction of aberrations that might arise. Building on this foundation, he then examines more advanced techniques such as quantitative fluorescence, fluorescence resonant energy, three-dimensional imaging, high-speed confocal microscopy, non-linear microscopy, and stimulated emission depletion.

Delivering a truly comprehensive work encompassing the scope and breadth of the field, the author brings a new level of understanding to the student, technician, researcher, or investigator working in the fascinating realm of optical microscopy.
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Pharmaceutical Microscopy 2011 Edition, Kindle Edition

Microscopy plays an integral role in the research and development of new medicines. Pharmaceutical Microscopy describes a wide variety of techniques together with numerous practical applications of importance in drug development. The first section presents general methods and applications with an emphasis on the physical science aspects. Techniques covered include optical crystallography, thermal microscopy, scanning electron microscopy, energy dispersive x-ray spectrometry, microspectroscopy (infrared and Raman), and particle size and shape by image analysis. The second section presents applications of these techniques to specific topics of pharmaceutical interest, including studies of polymorphism, particle size and shape analysis, and contaminant identification. Pharmaceutical Microscopy is designed for those scientists who must use these techniques to solve pharmaceutical problems but do not need to become expert microscopists. Consequently, each section has exercises designed to teach the reader how to use and apply the techniques in the book. Although the focus is on pharmaceutical development, workers in other fields such as food science and organic chemistry will also benefit from the discussion of techniques and the exercises.

  • Provides comprehensive coverage of key microscopy techniques used in pharmaceutical development
  • Helps the reader to solve specific problems in pharmaceutical quality assurance
  • Oriented and designed for pharmaceutical scientists who need to use microscopy but are not expert microscopists
  • Includes a large number of practical exercises to give the reader hands-on experience with the techniques
  • Written by an author with 21 years of experience in the pharmaceutical industry
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Quantitative Data Processing in Scanning Probe Microscopy: SPM Applications for Nanometrology (Micro and Nano Technologies) 1st Edition, Kindle Edition

Accurate measurement at the nano-scale – nanometrology – is a critical tool for advanced nanotechnology applications, where exact quantities and engineering precision are beyond the capabilities of traditional measuring techniques and instruments. Scanning Probe Microscopy (SPM) builds up a picture of a specimen by scanning with a physical probe; unrestrained by the wavelength of light or electrons, the resolution obtainable with this technique can resolve atoms. SPM instruments include the Atomic Force Microscope (AFM) and Scanning Tunneling Microscope (STM).

Despite tremendous advances in Scanning Probe Microscopy (SPM) over the last twenty years, its potential as a quantitative measurement tool have not been fully realized, due to challenges such as the complexity of tip/sample interaction. In this book, Petr Klapetek uses the latest research to unlock SPM as a toolkit for nanometrology in fields as diverse as nanotechnology, surface physics, materials engineering, thin film optics, and life sciences. Klapetek's considerable experience of Quantitive Data Processing, using software tools, enables him to not only explain the microscopy techniques, but also to demystify the analysis and interpretation of the data collected.

In addition to the essential principles and theory of SPM metrology, Klapetek provides readers with a number of worked examples to demonstrate typical ways of solving problems in SPM analysis. Source data for the examples as well as most of the described open source software tools are available on a companion website.

  • Unlocks the use of Scanning Probe Microscopy (SPM) for nanometrology applications in engineering, physics, life science and earth science settings.
  • Provides practical guidance regarding areas of difficulty such as tip/sample interaction and calibration – making metrology applications achievable.
  • Gives guidance on data collection and interpretation, including the use of software-based modeling (using applications that are mostly freely available).
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Theoretical and Applied Aspects of Systems Biology (Computational Biology Book 27) 1st Edition, Kindle Edition

This book presents the theoretical foundations of Systems Biology, as well as its application in studies on human hosts, pathogens and associated diseases.

This book presents several chapters written by renowned experts in the field. Some topics discussed in depth in this book include: computational modeling of multiresistant bacteria, systems biology of cancer, systems immunology, networks in systems biology.
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Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens: Volume Investigation of Biological Specimens (Cell Biology) 1st Edition, Kindle Edition

The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems.
  • Volume investigation
  • Three-dimensional reconstruction
  • Fluroescent probe design
  • Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology
  • Confocal data display and analysis
  • Twenty-eight pages of color
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David Border - Consultant Microbiologist

 

David Border - Consultant Microbiologist